Journal: PLoS ONE

Article Title: Rediscovering an old foe: Optimised molecular methods for DNA extraction and sequencing applications for fungarium specimens of powdery mildew (Erysiphales)

doi: 10.1371/journal.pone.0232535

Figure Lengend Snippet: DNA extraction protocols tested on five VPRI apple powdery mildew P . leucotricha specimens in this study.

Article Snippet: Wizard ® Genomic DNA Purification , WizG , Promega (A1120) , Precipitation.

Techniques: DNA Extraction, Extraction, Binding Assay, Purification, Lysis, DNA Purification

Journal: Scientific Reports

Article Title: The oncometabolite R -2-hydroxyglutarate activates NF-κB-dependent tumor-promoting stromal niche for acute myeloid leukemia cells

doi: 10.1038/srep32428

Figure Lengend Snippet: ( a ) Cell surface markers of StromaNKtert cells determined by flow cytometry. ( b ) StromaNKtert cells were treated with vehicle, 20 mM R -2HG or 1 mM octyl- R -2HG for 48 h and the intracellular level of R -2HG was quantified. ( c ) The 5-hydroxymethylcytosine level of genomic DNA was quantified using ELISA kit as described in the Methods. ** p < 0.01. ( d ) StromaNKtert cells were treated with vehicle, 20 mM R -2HG or 1 mM octyl- R -2HG and the transcription factor activity was determined by array-based analysis. * p < 0.05.

Article Snippet: StromaNKtert cells were collected and genomic DNA was extracted with Tissue & Cell Genomic DNA purification kit (GMbiolab Co. Ltd, Taiwan).

Techniques: Flow Cytometry, Enzyme-linked Immunosorbent Assay, Activity Assay